ABSTRACT. The elucidation of the resistance mechanism of weeds to herbicides is important for management practices. The objective of this work was to investigate the resistance mechanism of glyphosate-resistant C. sumatrensis biotypes by determining the expression levels of the constitutive gene epsps and two ABC transport protein-coding genes designated m7 and m11 with RT-qPCR. Two biotypes of C. sumatrensis were evaluated: one resistant and one susceptible to glyphosate. The treatments consisted of the absence or application of two doses of glyphosate (1,080 and 8,640 g a.e. ha-1). Plant leaves were collected at 1 and 4 days after herbicide application. No difference was observed in epsps gene expression between the studied biotypes. The expression of the m7 and m11 genes revealed that both genes had higher relative expression in the resistant biotype with the application of glyphosate at both doses. The overexpression of the m7 and m11 genes in the resistant biotype treated with glyphosate reveals that these genes play a role in herbicide resistance. These genes may be involved in the sequestration of glyphosate into the vacuole lumen in the resistant C. sumatrensis biotype studied.

Abstract Background: The pre-emergence herbicides diclosulam and flumioxazin are used to weed control in soybean. We hypothesized management of the new emergence of weeds in the early development stages of the soybean could alter the periods of interference. Thus, the period prior to interference allows and increases the canopy closure spaces before the critical period and disfavors the development of weeds inside the vegetative canopy. Objective: This study aimed to evaluate whether the period prior to interference of soybean cultivars was altered due to the application of residual pre-emergence herbicides. Methods: Two experiments were conducted with the soybean cultivars ‘NA 5909 RG’ and ‘P95R51’. One day before sowing in the main plots, the herbicide treatments paraquat (control without residual herbicide), paraquat+diclosulam, and paraquat+flumioxazin were applied. In the sub-plots, the increasing periods of coexistence of soybean with weeds of 0, 7, 14, 21, 28, 35, 42, and 49 days after the emergence of soybean were allocated. Results: The control without residual herbicide had the lowest period prior to interference and the most considerable reductions in grain yield when compared with residual herbicides in both experiments. The presence of residual herbicide increased the period prior to interference because they controlled the initial emergence of the major weeds in the experimental area. Conclusions: The use of the herbicides with residual activity diclosulam and flumioxazin increased the period prior to interference of ‘NA 5909 RG’ and ‘P95R51’ in areas infested mainly with horseweed and radish.

RESUMEN El manejo de raigrás es difícil debido a la ocurrencia de biotipos resistentes a diversos herbicidas con diferentes mecanismos de acción. Dado que la mezcla de herbicidas y sus rotaciones son una alternativa importante para el manejo de malezas resistentes, el objetivo de este trabajo fue evaluar la interacción de las dosis de los herbicidas glufosinato y atrazina para el control de raigrás y la calidad de sus semillas expuestas a su asociación. Se realizaron tres experimentos usando diseño factorial en condiciones de campo, laboratorio e invernadero. Dos factores (A y B) fueron evaluados en cada experimento, donde el factor A y B representaron las dosis de glufosinato y de atrazina, respectivamente. El control de raigrás fue evaluado en el experimento de campo, mientas que el porcentaje de germinación y el Índice de Velocidad de Emergencia (IVE) se determinaron en laboratorio e invernadero, respectivamente. Los datos fueron sometidos a análisis de la varianza (P≤0,05) y los resultados significativos fueron analizados a través de gráficos de superficie de respuesta. Para los datos de control, el efecto de las interacciones fue analizado por medio del método Colby; el glufosinato proporcionó un eficiente control de raigrás, pero su asociación con atrazina reduce su eficacia, siendo caracterizando como antagonismo entre moléculas. La aplicación del herbicida glufosinato, independiente de la presencia de atrazina, redujo la calidad de las semillas de raigrás en la fase de post-floración.

ABSTRACT Ryegrass management has been difficult by the occurrence of resistant biotypes to several herbicides with different action mechanisms. Since herbicides mixes and rotations are an important alternative for resistant weed management, the objective of this work was to evaluate the interaction of the dose of the herbicides glufosinate and atrazine on ryegrass control and its seeds’ quality exposed to their association. For this study, three experiments were carried out using factorial design in field, laboratory, and greenhouse conditions. Two factors (A and B) were evaluated in each experiment, where factor A and B represented the doses of glufosinate and atrazine, respectively. Ryegrass control was evaluated in field experiment, while germination percentage and Emergence Speed Index (ESI), were obtained in laboratory and greenhouse analyses, respectively. The data were submitted to variance analysis (P≤0.05) and the significant results were analyzed through response surface graphs. For ryegrass control data, the effect of the interaction was analyzed by the Colby method; glufosinate provides efficient ryegrass control, but its association with atrazine reduces the efficiency, being characterized as an antagonism between molecules. Glufosinate herbicide application, independent of atrazine presence, reduced the ryegrass seeds quality at the post-flowering stage.